No. MRC-Holland does not supply positive samples. You can sometimes obtain a suitable positive control from an online biorepository such as the Coriell Institute. This article lists positive samples that have been verified to be useful for specific MLPA probemixes.
DNA samples that are available from MRC-Holland can be divided in three categories: Binning DNA, Artificial Duplication DNA, and Reference Selection DNA.
- Binning DNA is intended to aid in correct peak assignment for mutation-specific probes so that an accurate bin set can be made for a probemix. Binning DNA should never be used as reference sample. A small amount of binning DNA is included with orders for most probemixes that contain a mutation specific probe.
- Artificial Duplication DNA contains artificially-made duplications for one or more probes in a specific probemix and is intended as positive control for validation purposes. The only available Artificial Duplication DNA is SD024, which can be used for the P002/P087 BRCA1, P045/P077/P090 BRCA2 probemixes.
- Reference Selection DNA is intended to help identify suitable reference samples that contain the expected allele copy number, but cannot be used as reference sample itself.
For a full list of available sample DNAs please visit our Sample DNA page.