MLPA is run on human DNA samples.
- A minimum of 50 ng of DNA in a 5 µl volume is required per MLPA reaction.
- DNA samples must be dissolved in TE0.1 (10 mM Tris-HCl pH 8.2 + 0.1 mM EDTA) or have added Tris-HCl pH 8.0-8.5 to a final concentration of 5-10 mM for sufficient buffering capacity during the initial denaturation step.
- Suitable DNA sample origin varies between MLPA probemixes but can be from blood, fresh healthy/tumour tissue, or FFPE healthy/tumour tissue.
The instructions for individual probemixes may differ; always read the probemix-specific product description for more details. For more on sample treatment and quality please visit our sample treatment page, and the assay setup instructions within the (MS-)MLPA General Protocol.