Multiplex Ligation-dependent Probe Amplification (MLPA) is a multiplex PCR-based method that can detect the copy number of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously, with results being available within 24 hours.

Besides copy number changes, MLPA allows the detection of selected point mutations. Furthermore, MLPA is able to detect methylation patterns in DNA when used in combination with a methylation-sensitive restriction enzyme (MS-MLPA). MLPA is used worldwide for diagnostics and research of human genetic disorders and tumours.

This brochure provides an overview of the MLPA and MS-MLPA techniques and some of their most popular applications. More information about our products can be found in one of our application-specific brochures or in our full product catalog.